2017 Vol.23 No. PP 119-123
IN VITRO CALLUS INDUCTION IN PHARMACOLOGICALLY SIGNIFICANT HERB: ECLIPTA ALBA
PUSHPLATA DODAKE1 AND *MALIKA PAL
1Department of Botany and Biotechnology,
Sadhu Vaswani College, BHOPAL (M.P.) INDIA.
*S.A.M. Girls College,
BHOPAL (M.P.) INDIA
*Corresponding Author :
Email: drmalikapal@gmail.com
ABSTRACT
A competent protocol was developed for in vitro plant regeneration and multiplication through callus culture of
Eclipta alba. Perfect medium for callus establishment through nodal tissue was observed in 2,4-D supplemented media.
The callus formed was creamish yellow in colour, showed a growth period of three to four weeks. A moderate quality
callus was obtained with different gradients of BAP supplemented medium. A white to creamy callus with rapid growth
was observed in the medium supplemented with NAA. Callus was subjected for multiple shoot induction.
Key words : Callus culture, Eclipta alba, In vitro, Regeneration.