2017 Vol.23 No.1 PP 63-68

EFFECT OF DIFFERENT MEDIA, TEMPERATURE AND HYDROGEN-ION ON THE GROWTH AND SPORULATION OF FUSARIUM OXYSPORUM F. SP. CICERI CAUSING CHICKPEA

^*B.D. S. NATHAWAT, O.P. SHARMA AND VIRENDRA KUMAR

Division of Plant Pathology,
Rajasthan Agricultural Research Institute,
Durgapura, (S.K.N.A. University, Jobner, JAIPUR -302018
*Corresponding Author : Email: dsnathawat@gmail.com

ABSTRACT

An experiment was conducted at division of Plant Pathology, Rajasthan Agricultural Research Institute, Durgapura, Jaipur (S.K.N.A.U, Jobner). In vitro studies were conducted to know the effect of different solid and liquid media, temperature and hydrogen-ion concentrations on sporulation and growth of Fusarium oxysporum f.sp. ciceri. The fungus grew the best on Potato Dextrose Agar media (90.00 mm) after 7 days of incubation at 25 ± 1ºC and significantly superior in comparison to other media tested. Among the different liquid media tested, maximum dry mycelium weight was recorded in Potato Dextrose Broth (472.75 mg) superior to all other liquid media tested. The most suitable pH level and temperature for growth of fungus was 6.0 (90.00 mm) and growth of F. oxysporum was maximum at 300C.

Key words : Biocontrol, Chickpea, Fusarium oxysporum f. sp. ciceri, Isolation.