2017 Vol.23 No.2 PP 415-422

INSECTICIDAL EFFICACY OF ENDOPHYTIC FUNGAL EXTRACT OF ANNONA SQUAMOSA AGAINST CALLOSOBRUCHUS CHINENSIS (COLEOPTERA: BRUCHIDAE)

P.N. JADHAV AND A.B. PARDESHI^*

P. G. Department of Zoology,
Deogiri College, AURANGABAD (M.S.) INDIA
*Corresponding Author : Email : pardeshianilkumar@gmail.com

ABSTRACT

Endophytic fungi are microorganisms that live inside the plant without causing any overt negative effect on plant tissues; rather they protect the host plant from pests and diseases. The insecticidal activity of endophytic fungal extract of leaf and seed of Annona squamosa against pulse beetle, Callosobruchus chinensis were studied. Endophytic fungi were isolated from the leaf and seed of Annona squamosa . The various concentrations of methanol and ethyl acetate crude endophytic fungal extracts were tested against Callosobruchus chinensis. The percent mortality was recorded after 96h.The insecticidal activity of the endophytic fungi isolated from leaf of Annona squamosa were (LD₁₀= 35.99mg/ kg, LD₅₀= 70.03mg/kg) in methanol and (LD₁₀= 70.57mg/kg, LD₅₀= 112.4mg/kg) in ethyl acetate respectively. The fungi isolated from seed of Annona squamosa were (LD₁₀= 12.46mg/kg, LD₅₀= 37.33mg/kg) in methanol and (LD₁₀= 33.72mg/ kg, LD₅₀= 65.11mg/kg) in ethyl acetate respectively. The mortality increases with increase in concentration of endophytic fungi. The methanol solvent extract showed more insecticidal property against C. chinensis due to endophytic secondary metabolites. Statistical variance, 95% confidence limits and regression equations are presented.

Key words : Annona squamosa, Callosobruchus chinensis, Endophytic fungi, Mortality.