2018 Vol.24 No.1 PP 53-57

BIODIVERSITICAL CHARACTERIZATION AND BIOCHEMICAL EVALUATION OF NATURALIZED SHRUB, ATI BALA (ABUTILON INDICUM) IN AGRA (UP) INDIA

ATUL TIWARI

Department of Biotechnology,
Dr. MPS College, SIKANDRA,
(Dr. B. R. Ambedkar University) AGRA, INDIA
*Corresponding Author E-mail : dratult@gmail.com

ABSTRACT

For the study of Biodiversity characterization and Biochemical analysis, the natural herb was transplanted at Botanical Garden, R.B.S. College, Agra. For the study of species diversity at four natural sites of Agra district, four formulae were used to study the maximum and minimum diversity of species at its natural sites. Shannon Wienner’s Index, Menhinick’s Index, Margalef’s Index value at four different sites was given in Table -1. These values determined the biodiversity variation at natural sites in Abutilon indium. The values of Moisture contents (%), Oil content (%) and Protein concentration (μ”/ml) in Abutilon indicum were also determined with variation at biochemical level Table -2. With the help of SDS-PAGE, in four samples of seeds of Abutilon indicum from four natural sites ( Bichpuri site -Lane 1, Kailash site -Lane 2, Kheria site - Lane 3, Chhalesar site - Lane 4). The total no. of protein bands showed diversity in Abutilon indicum plant in Table: 3. Shannon- Wiener’s Index, Menhinick’s Index, Margalei’s Index and Similarity Index (S) were applied to determine the distribution patterns and concentrations of wild species to pinpoint the diversity built-up and variation in the gene pool and identifying sites for in-situ conservation. The present work determined the status of diversity of experimental herb at particular four sites the need to conserve this diversity for current and future use.

Key words : Biochemical evaluation, Biodiversitical characterization, Shrub plant.