2018 Vol.24 No.1 PP 16-20

A MICROPROPAGATION TECHNIQUE OF HEMIDESMUS INDICUS (ASCLEPIADACEAE),A VALUABLE MEDICINAL PLANT

SUSANTA KUMAR MAITY

Department of Botany,
Govt. General Degree College, KESHIARY,
PASCHIM MEDINIPUR-721135 (WEST BENGAL) INDIA
*Corresponding Author E-mail: smaity.bot@gmail.com

ABSTRACT

An efficient protocol was established for in vitro clonal propagation of Hemidesmus indicus (Anantamul) belongs to the family Asclepiadaceae, a widely used medicinal plant through callus culture in using nodal segment. Yellowish nodular callus was observed from nodal segments on MS basal medium supplemented with 0.5 mg/L BAP + 0.2 mg/L NAA within four weeks of culture. Large number of shoots (11.4±0.2) and roots (8.2±0.4) were obtained when the callus was sub cultured on MS medium with 0.2 mg/L BAP. The regenerated plantlets were acclimatized by transferring them to soil. The survival rate of plantlets was found to be 90%. Regenerated plants were morphologically comparable having normal leaf shape and growth.

Key words : Callus culture; Hemidesmus indicus; Medicinal plant; Regeneration; Shoot proliferation.