2018 Vol.24 No.1 PP 27-32

EFFECT OF DIFFERENT CULTURE MEDIA ON CALLUS CULTURES OF SOME GENOTYPE OF MEDICAGO SATIVA

SHALINI JAIN

Department of Botany,
Government P.G. College,
GUNA (M.P.) INDIA
*Corresponding Author Email : drshaliniguna123@gmail.com

ABSTRACT

Eight genotypes of Lucerne as C-10, IG-1212, IL-75, A-3, LLC-9, LLC-3, Anand-2 and AL-95-12 obtained from Indian Grassland Fodder and Agroforestry Research Institute, Jhansi. This genotype observed the colour and texture of the callus from explants. The genotypic differences were found significant but the media differences were observed to be non-significant. The best callus quality in terms of callus colour was observed from both hypocotyl and epicotyls explant in genotype IG-1212 whereas the genotype AL-95-12 exhibited best callus colour quality from cotyledon explants. The best callus texture was observed in genotype Anand-2 and AL-95-12 from hypocotyls explants, in genotype C-10, IL-75 from epicotyl explant and in all the genotypes except LLC-3 in cotyledon explants. MS and SH medium supplemented with 2.0 mg/l 2,4-D, 1.0 mg/l NAA and 0.2 mg/l BAP in general showed the best callus quality from different explants.

Key words : Callus colour , Callus culture, Explants, Genotype, Lucerne and texture.