2020 Vol.26 No.1 PP 99-106
https://doi.org/10.33451/florafauna.v26i1pp99-106
Zinc induced leucocyte anomalies in the fresh water teleost Clarias batrachus
V. K. Verma1, *Neelima Gupta2 and D. K. Gupta3
1Department of Environmental Science,
Future Institute of Engineering and Technology,
BAREILLY(INDIA)
2C.S.J.M. University
KANPUR (INDIA)
3Department of Zoology,
Bareilly College, BAREILLY(INDIA)
*Corresponding Author :
Email : guptagrawal@rediffmail.com
ABSTRACT
Changes in Differential Leucocyte Count (DLC) were evaluated in the blood of the fish species Clarias batrachus and this was
used as an in situ indicator of the species’ exposure to zinc.Due to feeding and living in the aquatic environments, fish are
particularly vulnerable and are heavily exposed to pollution because they cannot escape from the detrimental effects of
pollutants. In the present study, the effects of zinc on the DLC(small and large lymphocytes, neutrophils, monocytes,
eosinphils and basophils) were examined under three different concentrations of zinc (1.00 ppm, 3.00 ppm and 6.00 ppm)
during 7, 14, 21 and 28 days exposure period. During examination, the percentage of both lymphocytes were significantly
lower as exposure time and concentration increased while in the case of neurtophils and monocytes, counts, were higher.
Eosinophils showed slight increase whereas basophils showed fluctuations in their results. The study of this research infers
that the essential metal in undesirable condition can affect the fish health. DLC represents a suitable biomarker of
environmental health and provides a tool for biomonitoring water quality.
Key words : C. batrachus, Differential Leucocyte Count, Zinc.