FLORA AND FAUNA ISSN 2456-9364(Online) 0971-6920(Print)
         
2020 Vol.26 No.2 PP 295-303
https://doi.org/10.33451/florafauna.v26i2pp295-303
Hydro-ethanolic extract of Gloriosa superba protecting acute splenic injury by modulating endogenous antioxidants pool in rats
Piyush Shukla, Naresh Kumar Sahu, Raj Kumar, Monika Bhadauria1 and *Satendra Kumar Nirala
Department of Rural Technology and Social Development
1Department of Zoology
Guru Ghasidas University,
Koni - BILASPUR-495009 (C.G.) INDIA
*Corresponding Author : Email : satendrak75@rediffmail.com
ABSTRACT
Present investigation was designed to evaluate hydro-ethanolic extract of roots of Gloriosa superba against combined exposure to alcohol and LPS induced splenic injury in rats. Thirty female albino rats were divided into five groups having six animals in each. Splenic injury was induced by combined exposure to alcohol and lipopolysaccharide (LPS) and 50, 100 and 200 mg/kg dose of extract of Gloriosa superba was evaluated for its efficacy. Combined exposure to alcohol and LPS significantly decreased total proteins but significantly increased triglycerides, cholesterol and lipid peroxidation process in spleen tissues, indicating metabolic perturbance and peroxidative cellular damage. Co-exposure to alcohol and LPS significantly decreased activity of superoxide dismutase, catalase, glutathione reductase, glutathione peroxidise, glucose-6-phosphate dehydrogenase as well as diminished reduced glutathione level in splenic tissue samples indicating exhaustion of endogenous antioxidant pool and development of oxidative stress. Conjoint administration of extract of Gloriosa superba at all the three doses offered significant protection in studied endpoints and maintained their values towards their respective control in dose dependent manner. It may be concluded that hydro-ethanolic extract of Gloriosa superba has potential to protect splenic injury by modulating endogenous antioxidants pool due to antioxidant phytochemicals present in extract.

Key words : Alcohol, Antioxidant pool, Gloriosa superba, Lipopolysaccharide (LPS), Oxidative stress.
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