2021 Vol.27 No.2 PP 177-184
https://doi.org/10.33451/florafauna.v27i2pp177-184
Phytochemical screening and antibacterial activity of essential oils of Cymbopogon citratus and Ocimum americanum : a comparative account
R. Mehta, Nishtha Yadaw, Naureen S. Khan1, K. K. Sen2 and *B. Chaurasia
Department of Rural Technology and Social Development,
Guru Ghasidas University,
BILASPUR (C.G.)-495009 INDIA
1Department of Botany,
2Department of Rural Technology,
Dr. C.V. Raman University,
KOTA- BILASPUR (C.G.) -495113 INDIA
*Corresponding Author :
Email : b.ch.myco@gmail.com
ABSTRACT
The present study highlights the extraction of essential oils from C. citratus and O. americanum using Microwave
Hydrodiffusion and Gravity method (MHG) and Hydrodistillation Method (HD), the qualitative and quantitative screening along
with determination of antibacterial potential. For the study, extraction of essential oils was performed using Microwave
Hydrodiffusion and Gravity method and Hydrodistillation Method. Comparative study was performed to check the total flavonoid
content (TFC), total phenolic content (TPC), biochemical test, thin layer chromatography (TLC) and determination of
antibacterial activity of the essential oils. The results of the study depicted that the MHG method for extraction of
essential oils was much better compared to extraction by HD method as supported by the biochemical tests, TFC, TPC and TLC
results. However, the essential oils did not show antibacterial activity. More study related to the antimicrobial activity
needs to be performed. Essential oils form an integral part of the pharmaceutical and healthcare industry hence, detailed
study regarding the isolation and application of essential oils is required. The present study suggests the use of MHG method
for extraction of essential oils.
Key words : Antibacterial activity, Cymbopogon citratus, Essential oils, Ocimum americanum, Phytochemicals.