2023 Vol. 29 No.2 PP 263-268
https://doi.org/10.33451/florafauna.v29i2pp263-268
Isolation of fungal endophytes at diferent pH from two wild plants of Mirzapur district (U.P.) India
Sneha Dwivedi, Ankita Rai, *Alok Kumar Singh, Manju Srivastava and Anand Prakash Singh1
Department of Botany, C.M.P. Degree College,
University of Allahabad, PRAYAGRAJ (UP), INDIA
1Department of Botany, Bipin Bihari College,
JHANSI (Affi. To Bundelkhand University, UP) INDIA
*Corresponding Author :
E-mail:dralokksingh1@gmail.com/div>
ABSTRACT
The impact of pH on the microbial colonisation of endophytes on two plants (Buchanania and Celastrus sp.) was studied.
The leaf and stem of two plant species were subjected to two PDA (Potato Dextrose Agar) medium at pH levels (4.5 and 9) for 5
weeks for isolation of fungal endophytes in aseptic condition. Over the testing period, pH 4.5 produced the highest fungal colony.
At different pH values, the fungal populations were noticeably different, with consistently higher counts at pH 4.5 and 9, respectively.
At pH 4.5 isolated fungi were Aspergillus niger, Alternaria, Curvularia, Fusarium, Rhizopus, Mucor, and Penicillium, and at pH 9.0
the isolated fungi were Aspergillus fumigatus, Penicillium, Alternaria, Curvularia, Fusarium, and Rhizopus respectively. It
demonstrates that varied pH levels led to changes in the fungal populations in plant species, leaves and stems (resistance/
susceptibility to environmental influences).
KEY WORDS : Buchanania, Celastrus, Colonization, Endophytes, Fungi, Leaf, Stem