2023 Vol. 29 No.2 PP 269-275
https://doi.org/10.33451/florafauna.v29i2pp269-275
Arsenic Cytotoxicity: A bioassay using Barley
*Vivek Singh and Manorama Singh1
Department of Botany,
Shri Jai Narain PG College,
LUCKNOW-226001 (U.P.) INDIA
1IGNOU Regional Centre,
LUCKNOW-226029 (U.P.) INDIA
*Corresponding Author :
E-mail:viveksingh_2@yahoo.com
ABSTRACT
Barley, Hordeum vulgare (2n=14) has been used by many cytologists, to determine cytotoxicity of environmental pollutants
like heavymetals, industrial effluents, agricultural chemicals etc. In the present study, Arsenic(in the form of arsenic trioxide of
concentrations100,250, 500,750and1000ppb), cytotoxicity was analyzed on Barley root tip mitosis, to understand its toxic effects
on cells and chromosomes of a plant-basedsystem. The results show that Arsenic can induce various Mito depressive and
clastogenic chromosomal aberrations like chromosomal breakages, disturbed spindle organization and other cellular metabolic
defects. Generally the cytological anomalies were found to be dose-dependent.
KEY WORDS : Aberrations, Arsenictrioxide, Cytotoxicity,Hordeum vulgare,Mito depression