2023 Vol. 29 No.2 PP 333-340
https://doi.org/10.33451/florafauna.v29i2pp333-340
In vitro use of Organic extracts of Tinospora cordifolia as anthelmintic against Fasciola gigantica larvae
Dharmendra Kumar Yadav1 and *Pradeep Kumar2
1Department of Zoology, S.G.N. Government P.G. College,
Muhammadabad, GOHANA, MAU-276403 (U.P.), INDIA
2Department of Zoology, Government Degree College
SUKRAULI, KUSHINAGAR-274207 (U.P.), INDIA
*Corresponding Author :
E-mail:pkumar_gpu@yahoo.co.in
ABSTRACT
This study aims to evaluate the in vitro anthelmintic larvicidal efficacy of Tinospora cordifolia against Fasciola gigantica
larvae (sporocyst, redia, and cercaria). Fascioliasis caused high economic loss in cattle as well as in the human population. It
can be controlled by the break of the life cycle of the Fasciola in the host snail Lymnaea acuminata. In vitro, exposure of dried
stem powder (DSP), various organic extracts, and column purified extract (CPE) of T. cordifolia was observed upto 8h exposure
at various concentrations against F. gigantica larva. The toxic effect of various preparations against larva was observed at 2, 4,
6, and 8h of the treatment. The column purified extract of dried stem powder of T. cordifolia against sporocyst, redia, and cercaria
in 2h LC50 was 50.27, 55.26, and 53.11 mg/L and 8h LC50 was 48.20, 48.37, and 48.12 mg/mL, respectively. Maximum effects of
column purified of T. cordifolia were observed against sporocyst larva (8h LC50 45.20 mg/L) of F. gigantica.
KEY WORDS : Cercaria, Fasciola, Redia, Snail, Sporocyst, Tinospora cordifolia.