2023 Vol. 29 No.2 PP 376-380
https://doi.org/10.33451/florafauna.v29i2pp376-380
Significance of N-acetyltranseferase-2 (NAT-2) gene polymorphism in the patients of cervical cancer
Balbir Singh, Dwijendra Nath1 and *Ramesh Kumar
Department of Biochemistry,
Bundelkhand University, JHANSI-284128 (U.P.) INDIA
1 Department of Pathology,
Maharani Laxmibai Medical College,
JHANSI-284128 (U.P.) INDIA
*Corresponding Author :
E-mail:cs.singh25@gmail.com
ABSTRACT
In concern of cervical cancer through the various screening and health care programmes running in Asian and Western
countries, it was established that the prevalence and mortality has declined tremendously. However cervical cancer is still
considered as one of the fetal and common cancer in females, followed by breast cancer and lung cancer in India. In the present
study, blood samples of confirmed cervical cancer patients through biopsy examination were collected and subjected for genomic
DNA isolation. Isolated DNA samples were amplified for detection of NAT-2 gene, subsequently gene polymorphism was detected
using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. We observed that NAT-2 gene
polymorphism was significantly associated with cervical cancer risk.
KEY WORDS : Amplification, Cervical cancer, Genomic DNA, NAT-2 gene polymorphism