2024 Vol. 30 No.1 PP 107-114

https://doi.org/10.33451/florafauna.v30i1pp107-114

The infection of Euclinostomum sp. in Channa punctatus with molecular with special and morphological study from Koderma reservoir, Jharkhand, India

Devnath Shukla, Akash Garain, *Anjana Verma and Bijay Kumar Behera¹

Vinoba Bhave University,
HAZARIBAG-825301 (JHARKHAND), INDIA
¹College of Fisheries,
Rani Lakshmi Bai Central Agricultural University,
Jhansi-Gwalior Road, JHANSI-284003, INDIA
*Corresponding Author : E-mail:bijay.behera@icar.gov.in

ABSTRACT

Worldwide, the parasite family Clinostomidae is widely dispersed. Euclinostomum sp., a common digenetic trematode, has a metacercariae stage that is ideal for infecting Channidae species because it becomes encysted in their kidneys, liver, and muscles. This paper aimed to identify Euclinostomum sp. in Channa punctata by means of a genetic and morphological combination of approaches. The morphological characteristics of the species were examined using both a light and scanning electron microscope. This parasitic organism was fascinatingly exposed by the SEM investigation, with its flattened, leaf-shaped body covered with surface characteristics. Sequencing the purified PCR results from many worms using Euclinostomum sp.’s 18sRNA gene produced sequences of 1700 bp nucleotides, on average. The NCBI has assigned Euclinostomum sp. the accession number OQ286054. The phylogenetic reconstruction demonstrated a strong genetic resemblance across the various strains of Euclinostomum, suggesting a common genetic ancestor. Compared to the monsoon and post-monsoon, the pre-monsoon had the highest percentage of parasite prevalence.

KEY WORDS : Channa punctatus, Euclinostomum sp, PCR, Prevalence, 18sRNA, SEM