2024 Vol. 30 No.1 PP 167-171

https://doi.org/10.33451/florafauna.v30i1pp167-171

GC-MS Analysis of bio-Active compound α-Terpinyl acetate extract of bay leaf having antimicrobial and antifungal properties

Vigyan Singh, *Gopal Prasad Agrawal and Balbir Singh¹

Institute of Pharmaceutical Research,
GLA University, MATHURA
MATHURA-281406 (U.P.) INDIA
¹Institute of Biomedical Sciences,
Bundelkhand University,
JHANSI (U.P.) INDIA
*Corresponding Author : E-mail:gopalprasad.agrawal@gla.ac.in

ABSTRACT

This study aimed to assess the phytotoxic and antibacterial properties of á-terpinyl acetate by evaluating the presence of T. pulegioides á-terpinyl acetate chemotype as a source of natural origin, which was examined in 131 sites by hydrodistillation, essential oils were separated, and GC-FID and GC-MS analyses were performed. The phytotoxic impact of this chemotype’s essential oil on monocotyledons and dicotyledons through water and air was investigated in a lab setting; The effect of the essential oil against pathogenic bacteria that affects humans was screened using the broth microdilution method. The findings indicated that á-terpinyl acetate was a very uncommon component in T. pulegioides essential oil, only being discovered in 35% of the habitats that were studied. On the plants under investigation, á-terpinyl acetate (both in essential oil and pure form) exhibited distinct behaviors.á-terpinyl acetate’s phytotoxic impact was more pronounced on the monocotyledons under investigation compared to the dicotyledons. For high economic productivity forage grass monocotyledon Poa pratensis, á-terpinyl acetate essential oil hindered seed germination and radicle development; while, for high economic productivity forage legume dicotyledon Trifolium pretense, it increased seed germination. The essential oil á-terpinyl acetate had a strong antibacterial action against dermatophytes and fungi, but a weaker impact against bacteria and Candida yeasts. Consequently, the á-terpinyl acetate chemotype of T. pulegioides may represent a promising chemical for the development of therapeutics or preventative measures against mycosis.

KEY WORDS : Antimicrobial effect, á-terpinyl acetate, Dicotyledons, Monocotyledons, Thymus pulegioides.