2024 Vol. 30 No.1 PP 172-176
https://doi.org/10.33451/florafauna.v30i1pp172-176
Mitochondrial antioxidants in normal oral epithelial and oral squamous cell carcinoma cell lines
Sumita Banerjee1, Shantirani Thokchom2, Sanjib Mitra3, Aditya Narayan4 and *Saikat Mukherjee2
Department of Oral Pathology,
1Department of Biochemistry,
Regional Institute of Medical Sciences,
IMPHAL (MANIPUR), INDIA
2Department of Oral Pathology,
Burdwan Dental College & Hospital,
BURDWAN (WB), INDIA
3Department of Zoology,
Bundelkhand University,
JHANSI (UP), INDIA
*Corresponding Author :
E-mail:mukherjeesaikat333@gmail.com
ABSTRACT
Mitochondrial antioxidants play an important role in regulation of oral cancer progression. In our study we profiled the
expression levels of various mitochondrial antioxidants such as reduced glutathione, lipid peroxides, mitochondrial catalase,
mitochondrial superoxide dismutase 2 (SOD2), and mitochondrial glutaredoxin 2( GLRX2) in normal oral epithelial cell line and
oral squamous cell carcinoma cell (OSCC) line. Our comparative study on these cell lines using UV-Vis Spectrophotometry and
Immunoblotting reveal there is an altered profile of the mitochondrial antioxidants in oral squamous cell carcinoma. While the
expression levels of mitochondrial Catalase, mitochondria Lipid Peroxide, mitochondrial Superoxide Dismutase 2 and mitochondrial
Glutaredoxin 2 decrease in oral squamous cell carcinoma cell line as compared to oral epithelial cell line, the levels of reduced
glutathione increase. Our results indicate there is a complex interplay of mitochondrial antioxidants in regulation of oral squamous
cell carcinoma.
KEY WORDS : Catalase; GLRX2; Lipid peroxide; Mitochondrial antioxidants; OSCC; Reduced glutathione; SOD2.