2024 Vol. 30 No. 2 PP 273-281
https://doi.org/10.33451/florafauna.v30i2pp273-281
Evaluation of antimicrobial and antagonistic activities of endophytic bacterial isolates of an ethnomedicinal plant of Manipur, Shamba (Oroxylum indicum)
*Heikham Kajal Devi, Shantirani Thokchom and Debananda S. Ningthoujam
1 *ICAR-Indian Institute of Sugarcane Research,
LUCKNOW (UP) INDIA 226 001
..
Biochemistry Department,
Manipur University,
CANCHIPUR-795003, IMPHAL (MANIPUR) INDIA
*Corresponding Author
E-mail: kajal.cdri@gmail.com
ABSTRACT
In the present study, a total of 57 endophytic bacterial isolates were obtained from leaf, stem and root samples of Oroxylum
indicum using Nutrient Agar (NA) and Starch Casein Nitrate Agar (SCNA). The isolates were screened for antibacterial activity
against a panel of test bacterial pathogens viz. Micrococcus luteus (MTCC 106), Bacillus subtilis (MTCC 121) and Escherichia
coli (MTCC 739) and also tested for antagonistic activity against fungal pathogen viz. Fusarium oxysporium (MTCC 287). Among
57 isolates, 5 isolates (OInL1, OInL6, OInL7, OInL11 and OInL14), 12 isolates (OInL12, OInL13, OInS1, OInS2, OInS13, OInS15,
OInS19, OInS20, OInR8, OInR12, OInR13 and OInR15) and 10 isolates (OInL1, OInL4, OInL7, OInL12, OInS15, OInS20, OInR13,
OInR15, OInR16 and OInR17) exhibited antibacterial activity against Micrococcus luteus (MTCC 106), Bacillus subtilis (MTCC
121) and Escherichia coli (MTCC 739) respectively in secondary antimicrobial screening using Kirby Bauer method. Furthermore,
among 57 isolates, 9 (OInL6, OInL7, OInL11, OInL12, OInL14, OInR13, OInR15, OInR16 and OInR17) showed antagonistic
activity against Fusarium oxysporium (MTCC 287).
KEY WORDS : Antibacterial activity, Antifungal activity, Endophytic bacteria, Oroxylum indicum