FLORA AND FAUNA ISSN 2456-9364(Online) 0971-6920(Print)
         
2024 Vol. 30 No.2 PP 195-205
https://doi.org/10.33451/florafauna.v30i2pp195-205
Evaluation of ethanolic and hexane extracts of Trechispora pallescens as antioxidant activity and anticancer potentiality against A549 cancer cell line
Tanmay Bera and *Swapan Kumar Ghosh
1 *ICAR-Indian Institute of Sugarcane Research,
LUCKNOW (UP) INDIA 226 001
. Molecular Mycopathology Lab,
Cancer Research Unit and Biocontrol Unit,
PG Department of Botany,
Ramakrishna Mission Vivekananda Centenary College (Autonomous),
City: RAHARA, KOLKATA-700118 (W.B.), INDIA
* Corresponding Author
E-mail : gswapan582@gmail.com
ABSTRACT
Trechispora pallescens (Bres.) K.H. Larse comb. nov. was collected and identified by molecularly (ITS) in West Bengal, India by us in our laboratory. In this article the research aimed to elucidate the antioxidant contents and properties of ethanol (TEE) and hexane (THE) extracts of Trechispora pallescens, along with assessing their potential anti-cancerous effects on lung cancer A549 cells line. The result showed that the extraction yields were 0.57 ± 0.03% for TEE and 0.72 ± 0.05% for THE. Phenolic content was significantly higher in TEE (44.33 ± 1.10 mg GAE/g) compared to THE (29.15 ± 0.79 mg GAE/g), whereas flavonoid content was slightly higher in TEE (25.9 ± 0.42 mg QE/g) than in THE (21.7 ± 0.14 mg QE/g). TEE also exhibited higher tannin content (11.78 ± 0.47 mg TAE/g) than THE (8.94 ± 0.39 mg TAE/g). Total antioxidant contents which determine the antioxidant capacity were found to be 98.56 ± 1.41 mg AAE/g for TEE and 67.11 ± 1.57 mg AAE/g for THE. Both extracts displayed concentration-dependent DPPH radical scavenging activity, with TEE exhibiting an EC50 of 8.25 ± 0.34 mg/mL and THE showed an EC50 of 9.82 ± 0.72 mg/mL, relative to ascorbic acid (EC50 = 0.0473 ± 0.0002 mg/mL). Cellular and nuclear morphological changes were noted on A549 cell line by treatment with both TEE and THE. In MTT assays, TEE and THE showed dose-dependent inhibition of growth of A549 cells, with TEE achieving IC50 values of 1402.16 ± 128.00 µg/mL (24 h) and 1173.03 ± 45.22 µg/mL (48 h), THE achieving IC50 values of 1887.45 ± 155.00 µg/mL (24 h) and 1732.11 ± 40.23 µg/mL (48 h). Percentage of apoptosis were dose dependent manners by both extracts (TEE and THE). Leakages of LDH from A549 cells were occurred by both extracts. It was a novel work because before this work none did work on antioxidant content, activity and anticancer potentiality of this mushroom. These findings certified the both extracts of T. pallescens as valuable sources of antioxidant and anticancer agents, suggesting their relevance for pharmaceutical and functional food applications.

KEY WORDS : A549 cell line, Anticancer activity, Antioxidant content, DPPH, Ethanolic extract, FRAP, Hexane extract, Lung cancer, Trechispora pallescens
Back
Feedback-Dr. A.K.Srivastav, Executive Editor,
Flora and Fauna,"Sukh Sadan" F-3, Surya Puram,
Sipri Bazar,Jhansi - 284003 (U.P.) India
Phone - 0510 - 2480424, 09415030098
E-mail : akscks@rediffmail.com
Copyright © 2008-2022. All rights reserved.
Sitemap
Visitor No : 738285
Designed & Maintained by
www.apvtechnologies.com