2024 Vol. 30 No.2 PP 395-404

https://doi.org/10.33451/florafauna.v30i2pp395-404

A phytochemicals based formulation induced faster wound healing of excision wounds in wistar rats

^*Lalita Kushwah¹, Rupali Dutt², Manisha Singh³, Sadhana Shrivastava⁴ and D.K. Sharma¹ The present study involved development and scientific validation of wound healing potential of three phytochemicalbased formulations at different concentration in experimental rat model. Formulation-I consisted of ethanolic extracts of T. erecta, T. procumbens, A. vera and C. longa. Formulation-II consisted of ethanolic extracts of A. indica, F. benghalensis, A. vera and C. longa. Formulation-III contained ingredients of both Formulations-I and II. The extracts were analyzed quantitatively, revealing that T. erecta had the highest content of total phenols (41.61±0.10 mg GAE/g) and flavonoids (52.50±0.53 mg QE/g) and tannins were found in T. procumbens (59.61±0.15 mg TC/g). Wound healing functions of formulations were assessed by topical application on excision wounds made in Wistar rats. The healing process was monitored by measuring the degree of wound contraction on alternate days. The polyherbal Formulation-III (5% concentration) was found to achieve complete wound contraction (Significant pd”0.0001) on 18th day in comparison to other Formulations. The study demonstrated that the Formulation-III was found to have the most potential in achieving rapid wound healing rate

KEY WORDS : Excision wound, Phytochemicals, Polyherbal formulation, Wound healing.