2025 Vol. 31 No.1 PP 131-142
https://doi.org/10.33451/florafauna.v31i1pp131-142
Phytochemical profiling and evaluation of antioxidant potential of Achyranthes aspera (prickly chaff flower) seed extract
S. Sathyamoorthy1, A. Sagaya Sowmya2 and *Rohit Seth1
1Cell Biology and Neuroscience Laboratory,
Department of Zoology,
School of Life Sciences, Guru Ghasidas Vishwavidyalaya (A Central University),
BILASPUR – 495009, (C.G.), INDIA.
2PG and Research Department of Zoology,
National College (Autonomous), Affiliated to Bharathidasan University,
TIRUCHIRAPPALLI – 620001, (T.N.), INDIA.
*Corresponding Author :
E-mail : sharmavarsha1980@gmail.com
ABSTRACT
The present study investigated the phytochemical profile and antioxidant potential of Achyranthes aspera (prickly chaff
flower) seed extract.Freshly mature dried seeds were collected from around Bilaspur, Chhattisgarh, India, and an ethanolic
extract was prepared using Soxhlet extraction.Qualitative phytochemical screening revealed the presence of multiple compounds,
including alkaloids, carbohydrates, proteins, amino acids, glycosides, steroids, saponins, tannins, flavonoids, and triterpenoids.
Antioxidant capacity was examined using three distinct radical scavenging assays: DPPH, ABTS, and H2O2. The ethanolic
extract demonstrated potent antioxidant effects, with IC50 values of 26.586, 28.2641, and 15.7931 μg/mL.Gas Chromatography-
Mass Spectrometry (GC-MS) analysis identified key bioactive compounds, such as n-hexadecanoic acid, oleic acid, and saponins,
contributing to the plant’s antioxidant, antimicrobial, anti-inflammatory, and anti-diabetic properties.High-Performance Liquid
Chromatography (HPLC) was used to quantify the presence of triterpenoid compounds in the extract.These findings suggest
that A. aspera seed extract is a rich source of bioactive compounds with significant antioxidant potential, supporting its traditional
use in herbal medicine, and indicating its potential for pharmaceutical development.
KEY WORDS : Achyranthes aspera, Antioxidant, GC-HR-MS, HPLC, Phytochemical